Preimplantation genetic diagnosis for infertile males with autosomal dominant polycystic kidney disease / 中华男科学杂志

<p><b>Objective</b>Autosomal dominant polycystic kidney disease (ADPKD) is one of the most common genetic renal diseases, which may cause oligoasthenospermia and azoospermia and result in male infertility. This study aimed to analyze the outcomes of preimplantation genetic diagnosis (PGD) in male patients with ADPKD-induced infertility.</p><p><b>METHODS</b>We retrospectively analyzed the clinical data on 7 male patients with ADPKD-induced infertility undergoing PGD from April 2015 to February 2017, including 6 cases of oligoasthenospermia and 1 case of obstructive azoospermia, all with the PKD1 gene heterozygous mutations. Following intracytoplasmic sperm injection (ICSI), we performed blastomere biopsy after 5 or 6 days of embryo culture and subjected the blastomeres to Sureplex whole-genome amplification, followed by haplotype linkage analysis, Sanger sequencing, array-based comparative genomic hybridization to assess the chromosomal ploidy of the unaffected embryos, and identification of the unaffected euploid embryos for transfer.</p><p><b>RESULTS</b>One PGD cycle was completed for each of the 7 patients. Totally, 26 blastocysts were developed, of which 12 were unaffected and diploid. Clinical pregnancies were achieved in 6 cases following 7 cycles of frozen embryo transplantation, which included 5 live births and 1 spontaneous abortion.</p><p><b>CONCLUSIONS</b>For males with ADPKD-induced infertility, PGD may contribute to high rates of clinical pregnancy and live birth and prevent ADPKD in the offspring as well. This finding is also meaningful for the ADPKD patients with normal fertility.</p>

Two years clinical observation of anterior all zirconia ceramic resin bonded fixed partial dentures / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To observe the restoration effect of anterior all zirconia ceramic resin bonded fixed partial dentures, and to find a new fixed restorative method for restoring one or two missing anterior teeth.</p><p><b>METHODS</b>Twenty-nine missing anterior teeth from twenty-three patients were restored with all zirconia ceramic resin bonded fixed partial dentures, and restorations were devided in to two groups according to the number of retainers, i.e. the single-retainer group and the two-retainer group. The integrity, secondary caries, marginal shade and color match of the restorations were evaluated with the standard of American Public Health Association (APHA). The average period of observation was 22 months.</p><p><b>RESULTS</b>One restoration from the two-retainer group failed, two restorations from the single-retainer group failed. The shade of two restorations were evaluated as discernable whiter compared to adjacent teeth. There was no secondary caries and marginal shade. The two year success rate of anterior all zirconia ceramic resin bonded fixed partial dentures was 90% (26/29).</p><p><b>CONCLUSIONS</b>All zirconia ceramic resin bonded fixed partial dentures used for less than two anterior missing teeth can achieved definite aesthetic effect and physiologic function. It was a new type of fixed restoration for anterior missing teeth. However, to determine long-term result of this restoration more data are needed.</p>

Association of TBX5 gene polymorphism with ventricular septal defect in the Chinese Han population / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Congenital heart disease is a diverse group of diseases determined by genetic and environmental factors. Considerable research has been done on genes associated with development of the heart. A recent focus is the role of transcription factor TBX5 in the development of atria, left ventricle and conduction system. As part of a larger study, high density, single nucleotide polymorphism (SNP) scanning was used to explore the relationship between TBX5 gene polymorphism and susceptibility to ventricular septal defect not associated with forelimb malformation in the Chinese Han population.</p><p><b>METHODS</b>One hundred and ninety two paediatric patients with congenital ventricular septal defect and 192 matched healthy control subjects were studied. The haplotype reconstructions were calculated by PHASE2.0 software. Haploview software was used to perform linkage disequilibrium assessment and defining of haplotype blocks. The algorithm used for defining of blocks was the confidence interval method.</p><p><b>RESULTS</b>The TBX5 gene region can be divided into 3 haplotype blocks of 27, 15 and 2 SNPs. Strong linkage disequilibrium exists within each block. SNP rs11067075 within the TBX5 gene had significant correlation with ventricular septal defect (P = 0.0037) by single marker association analysis. In addition, a 20 kb haplotype composed of 27 SNPs correlated with ventricular septal defect (P = 0.05, multiple loci regression analyses based on reconstructed haplotype blocks).</p><p><b>CONCLUSIONS</b>TBX5 is associated with the occurrence of ventricular septal defect and may be a predisposing gene to congenital heart disease in Han Chinese. This finding has set a direction for further genetic and functional studies.</p>

Ultrastructural study of sclerotic dentin surface following the application of the Er, Cr: YSGG laser / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the ultrastructure character of sclerotic dentin surface conditioned by the Er, Cr: YSGG laser and acid etching.</p><p><b>METHODS</b>Sixteen human teeth with sclerotic dentin were randomly assigned to 4 groups, and conditioned with acid etching (Group A), laser irradiation (Group B), laser irradiation after acid etching (Group C), or acid etching after laser irradiation (Group D) respectively. The characters of surface ultrastructure were observed using scanning electron microscope (SEM).</p><p><b>RESULTS</b>In Group A, the majority of dentin tubules were blocked by sclerotic rods. In Group B, the honeycomb-like change was found on the sclerotic dentin surface. The surface structure of Group C was similar to that of Group B. In group D etching wiped off the honeycomb-like structure.</p><p><b>CONCLUSIONS</b>The sclerotic dentin surface treated with laser irradiation turns into uniform honeycomb-like structure. The rough surface may be beneficial to bonding strength on sclerotic dentin after laser irradiation.</p>

Three-dimensional finite element analysis of two different framework designs of compound galvano-ceramic bridges / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To analyze stress distribution of two framework designs in compound galvano-ceramic bridges made with three-dimensional finite element models.</p><p><b>METHODS</b>Three-dimensional finite element models were developed for compound galvano-ceramic bridges with two designed retainers of Ni-Cr frameworks which ringed or partially covered the electroformed gold caps. The models were vertically loaded 200 N on the center of the occlusal surface of the pontic and loaded 400 N on the center of buccoclusal marginal ridge from buccal aspect to lingual aspect at a 30 degree angle with the occlusion plane respectively. The stress distributions and the level of stress concentration in the two models were analyzed by the FEA software.</p><p><b>RESULTS</b>Stress distributing in two designed framework of compound galvano-ceramic bridges were similar. The highest von Mises stress of compound galvano-ceramic bridges was concentrated in the distal connector of the pontic and retainer. Under the vertical loading, the von Mises stress was 24.17 MPa for the partial cover retainer of the Ni-Cr framework and 24.85 MPa for the circular retainer. Under the lateral loading, the corresponding figures were 42.83 MPa and 42.69 MPa. No matter vertical or lateral loading, the margin of electroformed cap was the concentration area of the von Miese stress.</p><p><b>CONCLUSIONS</b>In this study, the stress concentration area of compound galvano-ceramic bridges with Ni-Cr framework and electroformed gold caps was the connector and the margin of electroformed cap was the insubstantial area.</p>

Regeneration of transgenic Lespedeza thunbergii expressing levansucrase gene (Sac B) from Bacillus subtilis / 生物工程学报

The Bacillus subtilis Sac B gene with the vacuolar targeting signal sequence driven by 35S promotor was transferred into Lespedeza thunbergii by Agrobacterium mediated method. Total 62 Kan-resistant plants were obtained, of which 5 plants were proved to be transgenic plants. The transgenic plants were characterized by PCR amplification, PCR-Southern hybridization and RT-PCR. The physiological assay results showed that the transgenic plants were more tolerant to stress than the controls under the condition of 200mmol/L NaCl and 5% PEG, respectively, and that the content of soluble sugar in trnsgenic plants was significantly higher than that of controls in the period of tests (5-15 days) under salt and PEG stress.

Changes of upper airway of obstructive sleep apnea syndrome patients after the treatment of Silensor snoreguard / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the changes of upper airway of obstructive sleep apnea syndrome (OSAS) patients after the treatment of Silensor snoreguard.</p><p><b>METHODS</b>42 patients with OSAS were treated with dental appliance. Cephalometric analysis was carried out to compare the changes of upper airway before and after the treatment.</p><p><b>RESULTS</b>SPP-SPPW increased from (9.10 +/- 2.25) mm to (12.24 +/- 2.61) mm. PAS increased from (8.99 +/- 3.20) mm to (11.24 +/- 3.79) mm. CL decreased from (26.26 +/- 6.04) mm to (14.37 +/- 8.14) mm. H-MP decreased from (21.27 +/- 6.12) mm to (12.14 +/- 5.89) mm. V -RGn decreased from (55.15 +/- 5.61) mm to (51.63 +/- 5.87) mm. OPAA increased from (7.01 +/- 1.54) mm2 to (7.85 +/- 1.61) mm2. HPAA increased from (3.02 +/- 0.89) mm2 to (3.61 +/- 1.61)mm2.</p><p><b>CONCLUSION</b>Dental appliance worked with the effect of enlarging upper airway.</p>

Susceptibility patterns and mechanisms of macrolide resistance in group B streptococcus isolates / 中华儿科杂志

<p><b>OBJECTIVE</b>To test the antibiotic susceptibility and study mechanisms of macrolide resistance in group B streptococcus isolates (GBS).</p><p><b>METHODS</b>The GBS investigated in this study included 140 and 47 colonizing strains isolated from vaginal or cervical swabs from pregnant women in Beijing (from 1994 to 1999) and Guangzhou obstetrics and gynecology hospitals (from 1999) and 6 invasive strains isolated from infants in Beijing Children's Hospital. Susceptibility to ampicillin, penicillin G, erythromycin, lincomycin, cephazolin, cefuroxime, cefoperazone was assessed by K-B disc diffusion. The mechanisms, methylation or efflux, of macrolide resistant GBS isolates, were analyzed by PCR for ermB and mefA genes.</p><p><b>RESULTS</b>Susceptibility testing revealed that none of the GBS isolates were resistant to beta-lactam drugs, but 17% isolates showed intermediate susceptibility to penicillin G and ampicillin. The rate of erythromycin resistance increased from 8% in 1998 to 16% in 1999 in Beijing, while the rate of lincomycin resistance increased from 20% to 28% in that period. However, 21 (45%) and 12 (26%) isolates were resistant to erythromycin and lincomycin, respectively in Guangzhou city where erythromycin resistance rate was higher than that in Beijing. Of 45 erythromycin resistant isolates, 20 (20/45, 44%) possessed the ermB gene and 13 (13/45, 29%) harbored the mefA gene; 6 isolates harbored both genes, 6 isolates had possessed neither ermB gene nor mefA gene.</p><p><b>CONCLUSION</b>The susceptibility of GBS isolates to penicillin G and ampicillin suggests use of penicillin G or ampicillin as a first-line drug in prophylactic treatment regimes against early-onset neonatal GBS disease. Erythromycin and lincomycin should not be recommended as the second-line antimicrobial in Beijing and Guangzhou city. The clinical relevance of macrolide resistant GBS in women treated with macrolides for intrapartum prophylaxis needs to be assessed. Ribosomal modification by a methylase encoded by erm gene may play a major role in the mechanisms of macrolide resistance of GBS isolates in China.</p>

5-HT2C receptor subtype modulate production of secretory beta-amyloid protein precursor in incubated rat hippocampal slices / 药学学报

<p><b>AIM</b>To determine whether serotonin, a major neurotransmitter in brain, can modulate the production of secretory beta-amyloid protein precursor (sAPP) by activation of serotonin 5-HT2C receptor.</p><p><b>METHODS</b>The hippocampal slices of rats were incubated with various concentrations of serotonin, M-110, or L-107. sAPP released into the incubation medium were assayed by Western blot analysis assay with monoclonal antibody 22C11 for 2 h.</p><p><b>RESULTS</b>Various concentrations of serotonin (1.0 x 10(-2) - 1.0 x 10(3) micromol x L(-1)), M-110, a serotonin 5-HT2C agonist (1.5 x 10(-6) - 1.5 x 10(3) micromol x L(-1)), showed positive effect on the production of sAPP while L-107, a serotonin 5-HT2C antagonist (1.0 x 10(-9) - 1.0 x 10(3) micromol x L(-1)), showed negative effect on the production of sAPP over controls.</p><p><b>CONCLUSION</b>Serotonin modulates production of secretory amyloid beta-protein precursor through serotonin 5-HT2C receptor in incubated rat hippocampal slices.</p>

The expression of vascular endothelial growth factor, CD44v6 in non-small cell lung carcinoma / 中华外科杂志

<p><b>OBJECTIVE</b>To investigate the expression of vascular endothelial growth factor (VEGF), CD44v6 in non-small cell lung carcinoma.</p><p><b>METHODS</b>The expression of VEGF and CD44v6 in 35 cases I approximately II stages, 27 cases III approximately IV stages and 50 cases with the metastasis of lymph nodes, 12 cases without metastasis of non-small cell lung carcinoma (NSCLC) tissues were studied by SP immunohistochemistry staining, and the 3 years survival rates were calculated in 42 cases patients.</p><p><b>RESULTS</b>The positive rates of VEGF and CD44v6 in NSCLC were 73% and 69%, respectively. They were both higher than those of matched normal lung tissues, P < 0.01. The expression of VEGF and CD44v6 were related to clinical stages and metastasis of lymph nodes significantly. The expression rates of the two markers in NSCLC with the metastasis of lymph nodes were higher than those without metastasis, chi(2) = 7.146 and 5.376 respectively, and those of III approximately IV stages were higher than those of I approximately II Stages, chi(2) = 6.392 and 12.152 respectively. Survival rates of three years were lower to those patients with positive expression of the two makers than those with negative expression. In a multivariate analysis, besides TNM stages and metastasis of lymph nodes, the positive expression of CD44v6 and the positive expression of VEGF were also the independent prognostic factors to affect the Survival time.</p><p><b>CONCLUSION</b>The VEGF and CD44v6 protein may act as one of important indexes to indicate the process of infiltration and metastasis in NSCLC.</p>